ANTIMICROBIAL RESISTANCE AND MOLECULAR TYPING OF Salmonella spp. ISOLATED FROM POULTRY IN KHARTOUM STATE, SUDAN

Salmonella is considered one of the main foodborne pathogens. In this study a total 38 isolates were recovered from 679 (5.6 %) specimens collected within Khartoum State. The included, intestinal contents 11.4% (14/160) egg 7% (7/99), liver 5% (5/100) skin (10/200), feed 0% (0/40%), water (0/100).. Serotyping revealed presence eight serovars: Kentucky 11 (28.9%), Stanleyville 8 (21.05%), Virchow 6 (16%), Alachua 5 (13.16%), Blockley 4 (10.53%), Hadar 2 (5.26%), Typhimurium 1 (2.63%) and Havana (2.63%). Antibiotic resistance profile, using disc diffusion method, indicated that all sensitive to apramycin, chloramphenicol, cefoperazone cefotaxime. found be resistant tetracycline (52.6%), nalidixic acid (50.0%), compound sulfonamide (44.7%), sulfamethoxazole-trimethoprim (31.6%), streptomycin (26.3%), gentamycin (15.8%), neomycin furazolidone (7.9%), ampicillin (5.3%), ciprofloxacin amikacin (2.6%), amoxicillin-clavulanic ceftazidime colistin (2.6%) in decreasing order. Only 20 (52.6%) demonstrated multiple drug resistance. Four untypable by XbaI Pulsed Field Gel Electrophoresis (PFGE) technique however 33 typed differentiated into 22 PFGE patterns. This aimed determine prevalence, antimicrobial molecular typing spp. form poultry.